Gaoyuan Wang
China
Research Article
Effects of Cyclopamine on the Viability of Articular Chondrocytes in Rats with Adjuvant Arthritis In Vitro and Part Mechanisms
Author(s): Xianbing Song, Bao Zhang, Wang Taorong, Gaoyuan Wang, Yanping Huang, Ye Zhang, Meimei Liu and Xiaoyu ChenXianbing Song, Bao Zhang, Wang Taorong, Gaoyuan Wang, Yanping Huang, Ye Zhang, Meimei Liu and Xiaoyu Chen
The aim of the present study was to investigate the effect of cyclopamine, a hedgehog signaling pathway inhibitor, on Adjuvant Arthritis (AA), rat articular chondrocyte viability and part mechanisms in vitro. In this study, an AA rat model was established by Freund’s Complete Adjuvant (FCA), and the Arthritis Index (AI), secondary paw swelling degree and HE staining was used to evaluate whether the model was successfully established. Chondrocytes of the ankle joint of AA rats were cultured and identified. Cyclopamine (0, 0.03, 0.1, 0.3, 1, 3, 10 and 30 mg/L) was administered to determine chondrocyte viability. Chondrocyte apoptosis was detected by Annexin V-FITC/PI double dye. The expression of hedgehog signaling pathway-related proteins Shh, Ptch1 and Gli1 in chondrocytes was detected by western blotting. The results showed that AA was successfully induced by FCA, since the AI .. View More»
