Advanced Techniques in Biology & Medicine
Open Access
ISSN: 2379-1764
ISSN: 2379-1764
Harsha Hegde
India
Research Article
Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application
Author(s): Jigar N Jani, Suman Kumar Jha, Durga Singh Nagar, Rajveer Singh Chauhan and Harsha HegdeJigar N Jani, Suman Kumar Jha, Durga Singh Nagar, Rajveer Singh Chauhan and Harsha Hegde
This paper shows the potential of phloroglucinol for In Vitro regeneration and tuberization of microshoots of Tinospora cordifolia. Within a week of nodal explants culture, axillary bud in nodal segments sprouted in all the media investigated including basal MS media without any growth regulators. Maximum response (52.2 per cent) evinced by MS media supplemented with 6.98 μM Kin. The same media produced maximum response for multiple shoot formation out of total shoot bud sprouted fostering average 3 shoots with 3.9 cm shoot length and 4.2 number of leaves per shoot. Effect of PG also investigated for mass multiplication and In Vitro rooting. Basal MS+6.98 μM Kin+79.4 μM PG increased shoot bud induction from 52.2 per cent to 84.8 per cent and multiple shoot production per cent from 12.9 to as high as 60.3 percent and also supported maximum number of shoot per explants (7.5). T.. View More»
DOI:
10.4172/2379-1764.1000125