Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
James S. Malter
UT Southwestern Medical Center,
5323 Harry Hines Blvd, Dallas, TX 75390-9072
Tanzania
Research Article
Transforming Growth Factor-β1 Antagonizes Interleukin-5 Pro-Survival Signaling by Activating Calpain-1 in Primary Human Eosinophils
Author(s): Qifa Xie, Zhong-Jian Shen, Jiyoung Oh, Haiyan Chu and James S. MalterQifa Xie, Zhong-Jian Shen, Jiyoung Oh, Haiyan Chu and James S. Malter
Background: Eosinophils rapidly undergo apoptosis unless exposed to prosurvival cytokines such as interleukin 5 (IL-5) or granulocyte-macrophage colony stimulating factor (GM-CSF). In vivo, eosinophils are exposed to TGF-β 1 which can induce apoptosis suggesting it may function to counteract the effects of IL-5 or GM-CSF and limit, in vivo tissue eosinophilia.Objective: The objective of this study was to investigate the proapoptotic effects of TGF-β alone and in combination with IL-5 on eosinophils.Methods: Peripheral blood eosinophil (PBEos) viability was assessed using flow cytometry after exposure to TGF-β1 and IL-5. Calpain-1 activation was determined in cell extracts by western blot analysis of endogenous substrates and with a fluorogenic α-spectrin substrate. Molecular interactions between calpain.. View More»
DOI:
10.4172/2155-9899.S1-003