Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Karl-Dietrich Sievert
Universitat zu Lubeck Lubeck,
Germany
Research Article
In Vivo Biocompatibility Testing of a Collagen Cell Carrier Seeded with
Human Urothelial Cells in Rats
Author(s): Lisa Daum, Sabine Maurer, Martin Vaegler and Karl-Dietrich SievertLisa Daum, Sabine Maurer, Martin Vaegler and Karl-Dietrich Sievert
Tissue-engineered, matrix-stabilized autologous urothelium is a new option for urethral reconstruction, particularly for patients for whom other autologous grafts are not available. In vitro engineering of urothelial tissue requires biomaterials as cell carriers that increase the stability of cell-based implants. The aim of this study was to investigate a new highly standardized, industrially manufactured bovine collagen type I-based cell carrier (CCC) for its suitability as a carrier matrix for human urothelial cells (HUC). As an in vivo biocompatibility test the behaviour and degradation of these implants was to be proven in a nude rat model. Expanded HUC from tissue biopsies were phenotypically analysed by immuno¬cyto¬chemistry and seeded onto CCC. For in vivo application, CCC was seeded with PKH26-labelled HUC in high density and constructs were implanted onto the rectus a.. View More»
DOI:
10.4172/2157-7013.1000215