Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Majid AMSA
EMAN Testing and Research Laboratory, School of Pharmaceutical Sciences,
Minden 11800, Pulau Pinang
Malaysia
Research Article
Use of Nigella sativa Linn. Supercritical Carbon Dioxide Extract for Targeting the Angiogenesis Cascade
Author(s): Baharetha HM, Nassar ZD, Ahamed MK, Aisha AFA, Alfadly SO, Kadir MOA and Majid AMSABaharetha HM, Nassar ZD, Ahamed MK, Aisha AFA, Alfadly SO, Kadir MOA and Majid AMSA
The aim of this study was to investigate the antiangiogenic effect of the Nigella sativa seeds extracts. Twelve extracts were prepared from the N. sativa seeds by supper critical carbon dioxide by varying extraction pressure and temperature. GC-MS analysis revealed the presence of 22 compounds, mainly thymoquinone (TQ), thymohydroquinone and androstane-3,17-diol. The extracts showed significant antioxidant activity, and cell viability study on human umbilical vein endothelial cells (HUVECs) indicated that two extracts (A3 and B3), prepared at higher temperature (60°C) and the lower pressures (2500 and 3000 psi), were the most potent anti-proliferative extracts; the median inhibitory concentrations were 41.5 ± 1.9 and 60.2 ± 2.2 μg/mL, respectively. The extracts down-regulated the expression of vascular endothelial growth factor (VEGF) in endothelia cells causing i.. View More»
DOI:
10.4172/2167-0412.1000241