Journal of Carcinogenesis & Mutagenesis

Marco E M Peluso

Publications

• Research Article
  Exocycilic DNA Adducts in a Murine Model of Non-alcoholic Steatohepatitis
  Author(s): Marco E M Peluso, Armelle Munnia, Mirko Tarocchi, Mario Arciello, Clara Balsano, Roger W Giese and Andrea GalliMarco E M Peluso, Armelle Munnia, Mirko Tarocchi, Mario Arciello, Clara Balsano, Roger W Giese and Andrea Galli
  
  Abstract Introduction: Non-alcoholic fatty liver disease is the most common hepatic disorder in Western countries. The transition from abnormal accumulation of lipids toward non-alcoholic steatohepatitis (NASH) represents a key step in the development of chronic liver pathologies. Oxidative stress and lipid peroxidation have often been proposed as mechanisms in the progression to steatohepatitis. Methods: We have examined the hepatic levels of exocyclic DNA adducts, indicated from 3-(2-deoxy-β-Derythro- pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine (M1dG) adduct, a biomarker of oxidative stress and lipid peroxidation, in a murine model of NASH using the 32P-DNA postlabeling assay. Results: Our findings show that C57BL/6 mice fed with high-fat and cholesterol diet developed signs associated with NASH after eight weeks, whereas there was no evidence of steatos.. View More»
  DOI: 10.4172/2157-2518.S3-003

  Abstract PDF