Marianne S Brooks
Associate Professor, Department of Process Engineering and Applied Science, Dalhousie University, Halifax, Nova Scotia, B3J 2X4,
Canada
Research Article
Changes in Cell Structure, Morphology and Activity of Streptomyces venezuelae during the Growth, Shocking and Jadomycin Production
Stages
Author(s): Marianne S Brooks, Tracey J Burdock, Abdel E Ghaly and Deepika DaveMarianne S Brooks, Tracey J Burdock, Abdel E Ghaly and Deepika Dave
Streptomyces venezuelae have the ability to synthesize a group of novel benzoxazolophenanthridine antibiotics called jadomycin. The aim of the study was to investigate the changes in activity, cell structure and morphology of Streptomyces venezuelae while subjected to different environmental conditions during the growth, ethanol shocking, and jadomycin production stages. The activity of S. venezuelae was measured using the triphenyl tetrazolium chloride (TTC) technique while the microbial population was measured using the optical density and plate count techniques. Samples from each stage were viewed under the scanning electron microscope (SEM) and transmission electron microscope (TEM). The specific TF yield was calculated by dividing the TF yield by the number of cells. The specific TF yield remained constant at 2.44 x 10-8(μmol/CFU) during the growth period in nutrient rich medi.. View More»
DOI:
10.4172/1948-5948.1000073