Martins AMC
Brazil
Research Article
Cytotoxicity of Bothrops pauloensis Venom on Madin-Darby Canine Kidney Cells: Cell Death Mechanism
Author(s): Marinho AD, Morais ICO, Lima DB, Pessoa Bezerra de Menezes RRP, Pereira GJS, Silveira JAM, Toyama MH, Orzáez M, Martins AMC and Monteiro HSAMarinho AD, Morais ICO, Lima DB, Pessoa Bezerra de Menezes RRP, Pereira GJS, Silveira JAM, Toyama MH, Orzáez M, Martins AMC and Monteiro HSA
Bothrops is a genus of pit vipers (snakes) endemic to Central and South America. Snake envenomation (Bothrops genus) specifically Bothropoides pauloensis, a venomous snake popularly known as “Jararaca pintada”, which is found in the Brazilian territory or more commonly in the southwest of the state of Sao Paulois. Acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed the cytotoxic effect of Bothropoides pauloensis venom on MDCK cells, intracellular mitochondrial membrane potentiality (ΔΨm), BpV regulated Reactive Oxygen Species (ROS) and activation of caspases 3 and 7 in MDCK cells. MDCK cells were treated with different concentrations of B. pauloensis venom for 24 h and cell death were measured with annexin V and PI stain.. View More»
