Journal of Bone Research
Open Access
ISSN: 2572-4916
+44 1478 350008
ISSN: 2572-4916
+44 1478 350008
Payel Ghosh
India
Research Article
Differential Gene Expression Pattern in Osteoclast Precursor Cells of Indian Postmenopausal Women with and Without Osteoporosis: A Microarray Based Study
Author(s): Mehrunnisa M Raje, Suhas T Mhaske, Payel Ghosh, Mohan R Wani and Richa AshmaMehrunnisa M Raje, Suhas T Mhaske, Payel Ghosh, Mohan R Wani and Richa Ashma
Background: Osteoporosis is a multifactorial disease with strong genetic and epigenetic component. In spite of enormous candidate gene association studies, the etiology and molecular mechanism of disease is not fully known. For identification of new markers of osteoporosis which could be vital in diagnosis and prognosis of disease, genome-wide microarray expression approach was employed.
Methods: Osteoclast precursor cells were sorted from circulating monocytes of osteoporotic and nonosteoporotic post menopausal females with similar life-style and year after menopause. Following microarray experimentation, gene enrichment analysis was performed on significant DEGs using Database for Annotation, Visualization and Integrated Discovery (DAVID) tool. Top 10 novel genes were further used for construction of protein-protein interaction network using .. View More»
DOI:
10.4172/2572-4916.1000185