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Pereira SCL
Pereira SCL
Universidade Federal de Minas Gerais, Escola de Enfermagem, Departamento de Nutrição,
Belo Horizonte-MG
Brazil
Publications
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Research Article
Molecular Characterization of Klebsiella Isolates from Enteral Diets
Author(s): Pereira SCL and Vanetti MCDPereira SCL and Vanetti MCD
To evaluate the genotyping of Klebsiella isolates from enteral diets in public hospitals, as high-resolution support within epidemiological surveillance for controlling nosocomial infections. Methods: Klebsiella isolates were obtained from enteral diets at two public hospitals in the state of Minas Gerais, Brazil. These isolates were identified and serotyped. Total DNA of Klebsiella was extracted and subjected to three genotyping methods, to evaluate polymorphism between the strains and genetic diversity. Results: Twenty-one isolates of Klebsiella were obtained from the hospital enteral formulae; fifteen were identified as K. pneumoniae and six as K. oxytoca. The results from random amplified polymorphic DNA (RAPD) and 16S-23S rDNA analysis revealed high polymorphism among the K. pneumoniae iso.. View More»
DOI: 10.4172/2157-7110.1000528