Molecular Biology: Open Access

Refaat M

Publications

• Thesis
  C-Banding Karyotype and Molecular Characterization on Cumin, Caraway and Coriander
  Author(s): Ibrahim MA, Bekhit M, Neveen Hassan, Refaat M and El-Akkad TIbrahim MA, Bekhit M, Neveen Hassan, Refaat M and El-Akkad T
  
  Our goal for this study to characterize three species germplasm of family Apiaceae, namely (cumin, caraway and coriander), to make a chromosome characterization and molecular fingerprint for the mentioned species. Results performed on Karyomorphological showed that; Cuminum cyminum L. (cumin: 2n=14), Carum carvi (caraway: 2n=20) and Coriandrum sativum (coriander: 2n=22). The total Chromatin Relative Length percentage (RL%) showed cumin ± 10.69 μm ± 19.70 μm, caraway ± 7.30 μm ± 13.40 μm and coriander ± 5.58 μm ± 12.07 μm. Satellites in all the cases were associated to short arms. The molecular characterization for the three species (caraway, cumin, and coriander) was conducted using 5 AFLP combinations and 15 anchored-ISSR primers. The total amplified bands were 330 (162 ISSR+168 AFLP), with.. Read More»
  

  Abstract PDF