Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
+44 1478 350008
ISSN: 2329-9029
+44 1478 350008
Roy A
India
Research Article
Influence of Different Macro and Micro Nutrients on the Shoot Multiplication of Centella asiatica
Author(s): Krishnan ML, Roy A and Bharadvaja NKrishnan ML, Roy A and Bharadvaja N
The present study deals with the optimization of adventitious shoots cultures of Centella asiatica for maximum shoot proliferation under the influence of different nutrient manipulation. The effects of nitrogen source (NH4NO3/KNO3), carbon (sucrose), potassium source (KNO3), phosphorous source (KH2PO4), macronutrient (MgSO4) and micronutrient (MnSO4) of Murashige and Skoog (MS) media on shoot growth were investigated and these experiment treatments were supplemented with 1 mg/l 6-benzylaminopurine (BAP) plant hormones. The shoot growth was influenced by relative ratio of NH4+:NO3- with maximum shoot number (47.6 ± 1.52) in medium containing 100 mM (40 mM NH4NO3:60 mM KNO3) nitrogen source when NO3- concentration is higher than NH4+ ions. The MS media with 3% sucrose content showed optimum shoot multiplication (36 ± 1), as with increase in carbon concentration in the medi.. View More»
DOI:
10.4172/2329-9029.1000213