Journal of Data Mining in Genomics & Proteomics

Saavedra L

Publications

• Research Article
  Genome Mining and Transcriptional Analysis of Bacteriocin Genes in Enterococcus faecium CRL1879
  Author(s): Suárez N, Bonacina J, Hebert EM and Saavedra LSuárez N, Bonacina J, Hebert EM and Saavedra L
  
  Among 151 bacterial isolates from nine artisanal cheeses, Enterococcus faecium CRL 1879 showed antibacterial activity against the food-borne pathogen Listeria monocytogenes. The isolate produced a proteinase K-sensitive compound in the cell free supernatant. Genome analysis demonstrated the presence of enterocin A, enterocin B, enterocin P, enterocin SE-K4-like and enterocin X biosynthetic gene clusters. Nucleotide sequences encoding for a putative two-component bacteriocin were detected using bioinformatics tools, here named enterocin CRL1879αβ. A transcriptional analysis of all bacteriocin genes by quantitative real time PCR analysis (qRT-PCR) revealed the transcription of each enterocin gene at different levels. Finally, analysis of bacteriocin genes distribution in 251 E. faecium bioprojects was performed and compared to those identify in E. faecium CRL1879. The discrim.. View More»
  DOI: 10.4172/2153-0602.1000180

  Abstract PDF