Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Sezin Gürkan
Turkey
Research Article
Evaluation of Cytotoxic and Genotoxic Effects of Datura stramonium Extracts on Cultured Human Lymphocytes
Author(s): Zeynep Ülker Akal, Sezin Gürkan, Lokman Alpsoy and Abdulkadir YildizZeynep Ülker Akal, Sezin Gürkan, Lokman Alpsoy and Abdulkadir Yildiz
Datura stramonium is a plant in the Datura genus, within the solanaceae family. The active ingredients are
atropine, hyoscyamine (levorotary isomer of atropine) and scopolamine which are classified as deliriants, or
anticholinergics used as a hallucinogen and internally to treat madness, epilepsy, and depression for centuries. Also these have pharmacological potential with a cracking ability and are used in folklore medicine. In this study, our aim is to identify the major component of D. stramonium by using HPLC. Also we determine the cytotoxic (Lactate dehydrogenease, (LDH) and cell proliferation (WST-1)), genotoxic (sister chromatid exchange (SCE)) and apoptotic (TUNEL assay) effects of D. stramonium methanolic seeds extract (DE) on human lymphocytes culture by using different test assays. According to our HPLC results, DE has atropine (935 μg/ml) and scopolamine (.. View More»
DOI:
10.4172/2167-0412.1000170