Shekher Mohan
School of Pharmacy,
One John Marshall Drive, Huntington, WV, 25755
Tanzania
Research Article
Morphine-Mediated Cytoprotection against Hemin in SK-N-SH and A172 Cells
Author(s): Shekher Mohan, Tanner Robinson, Noah Allen, Lance Armstrong and Sarah StevensShekher Mohan, Tanner Robinson, Noah Allen, Lance Armstrong and Sarah Stevens
Study background: Heme and its catabolized form, hemin, are cytotoxic due to their ability to contribute to the production of reactive oxygen species, increase intracellular calcium levels and stimulate glutamate mediatedexcitotoxicity. Previous work has shown that activation of the opioid receptors (i.e., mu, kappa and delta) is neuroprotective against ischemia-induced neuronal death and neurotoxicity induced by A β oligomers in vitro. However, the role of these opioid receptor in hemin toxicity remains unclear. Activation of the Mu-receptor results in decreased 3, 5’-cyclic adenosine monophosphate (cAMP) and adenylyl cyclase activity, which then results in reduced cAMP-dependent Ca2+ influx. Therefore, we hypothesized that the activation of the opioid receptors by morphine may decrease hemin toxicity.
Methods: The hum.. View More»
