Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
+44 1223 790975
ISSN: 2155-9570
+44 1223 790975
Stefan Huber
Austria
Research Article
The Effect of Aluminium on the Morphologic Appearance, Viability and Phagocytic Activity of ARPE-19 Cells
Author(s): Gertrud Haas, Claus Zehetner, Stefan Huber, Yvonne Nowosielski, Nikolaos Bechrakis and Josef TrogerGertrud Haas, Claus Zehetner, Stefan Huber, Yvonne Nowosielski, Nikolaos Bechrakis and Josef Troger
Purpose: Aluminium is known to have toxic effects on the central nervous system. We wanted to explore the effects of aluminium on cultured ARPE-19 cells, in particular, changes in the morphologic appearance, viability and in the phagocytic activity of these cells. Methods: After addition of different concentrations of aluminium to the cell cultures, cellular morphology was evaluated by photomicrographs; viability was determined by mitochondrial activity measurement and phagocytosis by uptake of europium-labeled FluoSpheres. Results: Pretreatment of the cells with aluminium led to the formation of clots in the cell culture and there was a relative weak dose-dependent decrease in viability. However, phagocytic activity was severely impaired at each concentration with a peak decrease of 92.45% (± 8.21) at 1000 μmol... View More»
DOI:
10.4172/2155-9570.1000383