Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Urmil Tuteja
DRDE, Jhansi Road, Gwalior-474002
India
Research Article
Characterization of Immune Responses to Yersinia pestis (Indian Isolate) Infection in Mouse Model
Author(s): Shailendra K Verma, Lalit Batra, Thimmasandra N Athmaram, Prachi Pathak, Navya Katram, Gauri S Agrawal and Urmil TutejaShailendra K Verma, Lalit Batra, Thimmasandra N Athmaram, Prachi Pathak, Navya Katram, Gauri S Agrawal and Urmil Tuteja
Yersinia pestis, causative agent of plague, is one of the deadliest pathogens around globe. Innate immune response is first line of host defense against pathogens. Here, we have investigated innate and adaptive immune response in plague infected mice, gene expression levels of TLR1-9 and CD14, MyD88, NF-ĸB, TNF-α, MAPKp38, IL-1β were studied in peritoneal macrophages of plague infected mice in a time dependent manner (0 h, 24 h, 48 h, 72 h, 96 h and 120 h of post infection) by qRT-PCR. We also evaluated the immune response to Yersinia outer proteins (Yops) in Y. pestis infected mice. Selected genes (11 numbers) of Y. pestis encoding virulent factors viz, YpkA, YopH, YopM, V antigen, Pla, YopN, YopJ, YopE, YopK, F1 and pH6 antigen were amplified by PCR, cloned and expressed in Escherichia coli . To study the IgG.. View More»
DOI:
10.4172/2155-9899.1000151