Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Waaga-Gasser AM
Molecular Oncology and Immunology,
Germany
Research Article
Tumor Suppressor Gene P29ing4 is Overexpressed and Induces a CD8 T Effector Cell Response in Human Renal Cell Carcinoma
Author(s): Nichiporuk Stumpf E, Yeung M, Grimm MR, Grimmig T, Stern PL, Moench R, Lebedeva T, Pal S, Tripathi S, Bonventre JV, Chandraker A, Heemann U, Tsaur I, Blaheta R, Lissner R, Germer CT, Riedmiller H, Gasser M and Waaga-Gasser AMNichiporuk Stumpf E, Yeung M, Grimm MR, Grimmig T, Stern PL, Moench R, Lebedeva T, Pal S, Tripathi S, Bonventre JV, Chandraker A, Heemann U, Tsaur I, Blaheta R, Lissner R, Germer CT, Riedmiller H, Gasser M and Waaga-Gasser AM
Objective: ING1 and ING4 are identified as candidate tumor suppressor genes acting in regulation of DNA damage responses and apoptosis through modulation of p53. Their defective function promotes tumor growth in melanoma and breast cancer. Our aim was to determine the overexpression of relevant p33ING1b and p29ING4 isoforms in patients with Renal Cell Cancer (RCC).
Methods: Peripheral Blood Mononuclear Cells (PBMCs) from tumor patients (Robson stage I-IV) were stimulated with overlapping peptides of p33ING1b/p29ING4 and results were compared with expression profiles in primary tumors.
Results: Early-stage and late-stage tumors demonstrated upregulated ING-isoform gene and protein expression. Early cancers were characterized by increased CD8 and IFN-γ protein and gene expression. Significant p33ING1b and p29ING4 tum.. View More»
DOI:
10.4172/2155-9899.1000511