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Biosensors & Bioelectronics

ISSN: 2155-6210

Open Access

Wai Kwan Lim


Singapore

Publications
  • Research Article
    Immunosensor Characterization Using Impedance Spectroscopy
    Author(s): Lin Ke, Wai Kwan Lim, New Siu Yee, Xiao Di Su and Bin LiuLin Ke, Wai Kwan Lim, New Siu Yee, Xiao Di Su and Bin Liu

    Non-invasive and high sensitive electrochemical impedance spectroscopy (EIS) has been used for qualitative characterization of stepwise surface preparation process for constructing an immunosensor, starting from plain silicon substrate, 3-aminopropyltriethoxysilane (APTES) silanization, glutaraldehyde functionalization and analytes (rat immunoglobulin G immobilization, followed by protein G-coated gold nanoparticles), using both the horizontal direction (2 terminal) and vertical direction (3 terminal) characterization setup. The investigation demonstrated that 2-terminal setup is able to review the quality of antibody and antigen molecules bonding and distinguish the effects from the underlayers on the immuosensors; while 3-terminal setup is able to correlate the thickness and dielectric constant of each layers. It correlated well to the incubation time of preparing different sensors .. Read More»
    DOI: 10.4172/2155-6210.1000138

    Abstract PDF

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