Applied Microbiology: Open Access
Open Access
ISSN: 2471-9315
+44 1300 500008
ISSN: 2471-9315
+44 1300 500008
Yéra H
France
Research Article
Diagnosis of Intestinal Parasitoses: Comparison of Two Commercial Methods for Faecal Concentration Using a Polyparasitized Artificial Liquid Stool
Author(s): Paugam A, Ngamada F, Eldin De Pécoulas and Yéra HPaugam A, Ngamada F, Eldin De Pécoulas and Yéra H
As conventional faecal concentration methods for parasite detection may carry health risks due to the toxicity of the solvents used (ether, acetyl-acetate), we compared parasite recovery obtained with the new solvent-free EasyPara® kit (Servisol, Meudon, France), which consists of a single-use tube containing a porosity gradient filter ( 200 to 400 μm), and that obtained with the Para-Selles® system (Fumouze Diagnostics/Sofibel, Levallois-Perret, France), an ethyl-acetate solvent sedimentation method routinely used in our laboratory. Both kits were used as recommended by their manufacturers. Both kits concentrate parasites in a pellet, which is suspended for microscopic examination. Parasites were identified on the basis of their morphology. The numbers of parasites recovered in the total pellets were compared between the kits. To compare the detection thresholds of the kit.. View More»
DOI:
10.4172/2471-9315.1000108