Journal of Bioprocessing & Biotechniques

Yu Ji

Publications

• Research Article
  Mathematical Modelling of Protein Precipitation Based on the Phase Equilibrium for an Antibody Fragment from E. coli Lysis
  Author(s): Yu Ji and Yuhong ZhouYu Ji and Yuhong Zhou
  
  Precipitation is an important operation in biopharmaceutical purification yet the mechanism of protein precipitation in multi-component solutions is not well understood. Existing models lack fundamental understanding of the process. In this paper, a new model describing how the protein solubility changes in the protein precipitation is proposed and is based on the phase equilibrium of the light liquid phase and dense solid phase. The model structure is generic and robust. It adequately reflects the non-linearity of protein precipitation kinetics and thus provides new fundamental insights into the protein precipitation in multi-component, complex protein solution. Two feed stocks of a pure fragment antigen-binding (Fab’) solution obtained by chromatographic purification and a clarified Fab’ homogenate solution from E. coli were used to examine the effect of ammonium .. Read More»
  DOI: 10.4172/2155-9821.1000129

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