Journal of Glycobiology
Open Access
ISSN: 2168-958X
+44 1478 350008
ISSN: 2168-958X
+44 1478 350008
Yukio Yuzawa
Japan
Research Article
Development of a Polyclonal Antibody Against Synthetic Human Immunoglobulin A1 Hinge Glycopeptide
Author(s): Koichiro Yamamoto, Hideo Hori, Yoshihiro Yamamoto, Kazuo Takahashi, Yukio Yuzawa and Yoshiyuki HikiKoichiro Yamamoto, Hideo Hori, Yoshihiro Yamamoto, Kazuo Takahashi, Yukio Yuzawa and Yoshiyuki Hiki
Background: Undergalactosylated IgA1 has been found to be increased in IgA nephropathy (IgAN) by an ELISA assay using Helix aspersa agglutinin (HAA) that recognizes N-acetylgalactosamine (GalNAc). In this study, we developed a polyclonal antibody (anti-sHGP antibody) against a synthetic IgA1 hinge peptide with five GalNAc residues.
Methods: The specificity of the anti-sHGP antibody was evaluated through the incremental treatment of IgA with corresponding glycosidases. Then, the susceptibility of the IgA to anti-sHGP antibody was compared among IgAN patients (n=39), patients with other forms of kidney diseases (OKD, n= 36) and healthy controls (n=37), using ELISA assay. The association of the binding abilities between anti-sHGP antibody and HAA were evaluated blindly using same 85 sera.
Results: The binding ability of t.. View More»