Gastroenteritis is a major, but a substantially neglected, public health problem and it can be caused by different bacterial, viral and parasitic pathogens. The predominant viral pathogens are rotavirus, norovirus, enteric adenovirus (ADV), astrovirus, coronavirus, Coxsackie virus, and ECHO virus. Real-time PCR for detecting viruses associated with gastroenteritis has been described as a convenient, useful, and powerful diagnostic tool, while multiplex assays of more than one viral gene target in a single tube have the advantage of rapid screening of a large number of potential pathogens in a short time.
Chen D, Sun Y, Zhao L, Qian Y, Zhu R, et al. (2014) Development of Two Multiplex Real-Time PCR Assays for the Rapid Detection of RNA and DNA Viruses Associated with Gastroenteritis in Pediatric Patients. Pediat Therapeut 4: 208.