Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
The effect of substrate concentration, pH, temperature and inhibitor (silver nitrate) on the activity of the enzyme invertase was studied using sucrose as the substrate. The invertase acted on the sucrose and converted it to product, glucose and fructose. From Lineweaver-Burk Plot, Vmax was found to be 1 mM/min and the Km approximately 24 mM. At temperature range of 25ËC to 650ËC, the optimum activity was at 450ËC. For a pH range of 3.5-6.0, the pH optimum activity was at 5.5. Inhibition studies showed that silver nitrate inhibited invertase competitively with apparent Km of 150 mM and Ki value of 160 mM.