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Effect of pressure, surface tension and rest potential on intra organelle nanoporation of multi layer osteoblast cell placed in a 3D non uniform micro fluidic chip composed of bi metallic heterogeneous micro electrode under the influences of smart control FPGA based Pico pulse generator. It is exposed that all above parameters are not uniformly distributed over the surface and affects the pore free energy. Pore radius and density are symmetric about the equator with the same value at both poles of the cell. In addition, the value of the rest potential and surface tension affect membrane potential around the cell because the electro oration current is several orders of magnitude larger than the ionic current that supports the rest potential and surface tension.