alexa Development and use of Bacteroides 16S rRNA Polymerase Chain Reaction Assay for Source Tracking Dog Faecal Pollution in Bathing Waters

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Development and use of Bacteroides 16S rRNA Polymerase Chain Reaction Assay for Source Tracking Dog Faecal Pollution in Bathing Waters

Faecal indicator bacteria (FIB) such as Escherichia coli (E. coli) and Enterococci are currently used to determine faecal bathing water pollution; they are found in a variety of warm-blooded animals and are not unique to the intestinal flora of humans [1]. Determining the exact sources of faecal pollution is now of critical importance when attempting to comply with the EU bathing water directive 2006 [2]. Bacteria belonging to the genus Bacteroides are now used as additional source-tracking indicator bacteria, since they constitute a major part of the faecal bacterial population; as strict anaerobes they have little potential for growth in bathing waters and have a high degree of host specificity [3,4].

 
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