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The presence of erythropoietin receptors and erythropoiesis on erythrocytes is disputable. However, the specific binding of erythropoietin to mammalian erythrocytes was reported as well as gene expressions. Recently, we proposed a growth-division and fission process involving adult human erythrocytes. Normal adult human erythrocytes were isolated from the blood of the authors collected in EDTA-Na2 tubes by venipuncture. Erythrocytes were separated by centrifugation at 400 g for 10 minutes. A total of 50 μl of packed red blood cells was suspended in 10 ml of DMEM/F12, and then cultured in disposable conical tubes, then examined on days 0, 7, 14, 21, 28, 35 and 42 of culture at room temperature.