alexa Division and Budding of Human Erythrocytes in Serum-Free Cultures

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Division and Budding of Human Erythrocytes in Serum-Free Cultures

The presence of erythropoietin receptors and erythropoiesis on erythrocytes is disputable. However, the specific binding of erythropoietin to mammalian erythrocytes was reported as well as gene expressions. Recently, we proposed a growth-division and fission process involving adult human erythrocytes. Normal adult human erythrocytes were isolated from the blood of the authors collected in EDTA-Na2 tubes by venipuncture. Erythrocytes were separated by centrifugation at 400 g for 10 minutes. A total of 50 μl of packed red blood cells was suspended in 10 ml of DMEM/F12, and then cultured in disposable conical tubes, then examined on days 0, 7, 14, 21, 28, 35 and 42 of culture at room temperature.

 

Citation: Niimi G (2013) Phase of Division and Budding of Human Erythrocytes in Serum-Free Cultures and Further Refinement Mass Production Plan by Amphiphilic Surfactants. Human Genet Embryol 3:104.

 
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