alexa Fish Medicine

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Fish Medicine

Evaluation of a Flow-Through Depuration System to Eliminate the Human Pathogen Vibrio Vulnificus from Oysters

he reaction mixture for the first/single step PCR contained crude DNA extract (template DNA), reaction buffer (100mM Tris with 15mM MgCl2), 0.4µg each of forward and reverse primer (1s5 & 1a16; IK1 & IK2), 100mM each dNTPs (deoxyribonucleotide triphosphates), 3U Taq DNA polymerase and molecular grade water. The components were mixed thoroughly and the PCR reaction was conducted in the Thermalcycler (GeneAmp 9700, ABI Systems, Rotkreuz, Switzerland). The PCR protocol comprised of 35 cycles of 60 sec at 94°C, 60 sec at 55°C and 90 sec at 72°C. The programme included an initial delay of 4 min at 94°C and final extension of 5 min at 72°C before and after 35 cycles, respectively.

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