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Matrix assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS) has been introduced several years ago as a new method for bacterial identification. Since a couple of years a variety of studies have been investigated the accuracy of a MALDI-TOF MS-based identification of grown colonies. Since proteomic profiles were compared to a database, the accuracy of the identification results on the database used. For most identification approaches, protein peaks in the mass-to charge ratio of 2000 to 10000 Da were used, most of them thought to represent ribosomal protein peaks. The results of commercial databases are believed to be very high in accuracy. Nevertheless, an independent protein profile deposition possibility and further optimization strategies are desirable in order to improve these databases independently of the manufacturer. Therefore studies with focus on the accuracy of identification of rare bacteria, fungi and other organisms are highly appreciated.
Citation: Florian S (2012) Whole-Cell Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry Applications in Medical Microbiology. J Med Microb Diagn 2:e111
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