alexa The TGF-and#946;1-Induced Expression of Matrix Metalloproteinases

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The TGF-β1-Induced Expression of Matrix Metalloproteinases

Transforming growth factor (TGF)-?1 activates the expression of matrix metalloproteinases (MMPs) in fibroblasts. Attachment of these cells to laminin-111 further raises the TGF-?1-induced expression of MMP-3 and MMP-10. Mesenchymal stromal cells (MSC) attach to a variety of extracellular matrix proteins during development and wound healing. We therefore investigated the TGF-?1-regulated expression of MMPs in MSC upon attachment to laminin-111 and type I collagen. The expression of MMPs was determined by quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. The TGF-?1 signalling pathways were investigated by immunoblot and by pharmacological blocking of Smad2, MEK/ERK and p38MAPK activities. Overall, TGF-?1 significantly activated the expression of mRNA encoding MMP-3 (p=0.05), MMP-13 (p=0.05) and TIMP-1 (p=0.01) in MSC.

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