RUNX2 was an essential for osteoblast differentiation and bone development. In our study, rat adipose-derived stem cells (ADSCs) were isolated from fat pads of abdomen and inguinal, and then purified and expanded in vitro. RUNX2 gene was over-expressed using lentivirus tools to induce the rat ADSCs differentiated into osteoblasts. The result shown that the transcription factors, RUNX2 could stable express in rat ADSCs and promote the directly osteoblasts differentiation of rat ADSCs. Western blotting assays revealed the up-regulation of osteoblast-related genes after osteoblastic differentiation of rat ADSCs. This study provides a theoretical basis and experimental evidence for the application of ADSCs into treatment of bone injury.
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