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Prebiotics have been found to have prophylactic potentials against colon cancer. Thus, the present study was designed to delineate the prophylactic role of prebiotics in experimental colon carcinogenesis. It was found that inulin+DMH-treated mice had significantly increased (p<0.05) body mass till 5th week and thereafter, decreased while in lactulose+DMH-treated mice the body mass increased significantly (p<0.05) from 5th week till end of experiment compared with DMH-only treated mice. Further, it was observed that mice belonging to inulin+DMH and lactulose+DMH-treated groups had significantly (p<0.05) higher lactobacilli counts compared with DMH-only-treated mice.