Polyacrylamide Gel is used in different fields of biology used for separating biological macromolecules such as protein and nucleic acids. This gel as specific properties such as chemically inert, electrically neutral, hydrophilic and transparent. It can detect at wavelengths greater than 250 nm. It has greater resolving power, which can accommodate larger quantities of DNA without significant loss in resolution and the DNA recovered from this gel is extremely pure. In addition, the matrix does not interact with the solutes and has a low affinity for common protein stains.