Bioprospecting activities tap vast sources of microbes from diverse and extreme habitats. Microbial hydrolases are important in the development of sustainable biomass energy or biofuels. To achieve full potential of this resource, there is a need to discover novel, unexploited microbes and their enzymes. So, a bioprospecting survey was conducted to identify fungal sources of biomass hydrolysing enzymes from different environments. Out of the total isolates, 39 were found to exhibit cellulolytic, hemicellulolytic and ligninolytic activities.