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Effects of Exogenous Melatonin in Clarias Macrocephalus Male Broodstock First Puberty Stage
The purpose of this study was to investigate the exogenous melatonin feeding administration to the first puberty stage in male broodstock of the Walking catfish, Clarias macrocephalus. The melatonin level of 0 (Control), 50 (Mt0.05) and 250 (Mt0.25) mg/kg in the diet mixed in isonitrogenous and isocaloric of 37% crude protein and 9.3% crude lipid was applied. The male maturation analysis for this study comprised of gonad histology, testosterone assay, gonadosomatic index, sperm abnormality, live sperm rate, sperm concentration, and sperm kinetic parameters.