Regulation of seed germination is quite complex and is further complicated by interaction of hormones like gibberellin (GA), abscisic acid (ABA) and ethylene. Moreover, the involvement of reactive oxygen species (ROS) in hormone signaling for such regulation is still less understood. The aim of the present study was to explore the interactive role of GA, ABA and ethylene and possible involvement of ROS in mediation of hormone action during seed germination of Vigna radiata. Seeds of V. radiata were germinated in presence of hormones, their biosynthesisor action inhibitors and in combinations with hydrogen peroxide and at intervals germination percentages were determined. Treated seeds were also tested for production of superoxide by NBT staining and analysed for NADPH oxidase (NOX) activity by in-gel assay.
For more information: Chaudhuri A, Singh KL, Kar RK (2013) Interaction of Hormones with Reactive Oxygen Species in Regulating Seed Germination of Vigna radiata (L.) Wilczek. J Plant Biochem Physiol 1:103