Mastitis is an important disease of dairy cattle. There are two formsof mastitis. One is clinical and another is sub clinical. Clinical Mastitisis easily detected by its clinical signs and milk quality but sub clinicalmastitis shows no lesions. Other hand subclinical mastitis cause most economic losses compared to clinical form. Thus rapid field detection technique is a strong tool to reduce this form. California Mastitis Test (CMT) is the most quick and reliable field test to detect this form of mastitis.