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Ensiling of agricultural straw produces feed with low protein content. To get high protein feed with high production and low cost, a two-step solid state fermentation was designed and several factors were optimized to maximize the yield. Candida tropicalis was first inoculated sweet sorghum stalk under aerobic condition for cell growth and protein content (PC) in the substrate after the first-step reached 26.3%. In the second-step, Lactobacillus rhamnosus was inoculated the sterilized substrate harvested from the first step, and cultured under anaerobic condition.