Double handling of hatching eggs by nanostructured complex of the biologically active compounds (ethanolamine, succinic acid and serine) significantly influenced embryogenesis of poultry. This handling has a positive influence on the state of their liver. These results can be explained by decreasing degree of lipid peroxidation and increasing capacity of the “antioxidant defense” system. Peroxidase activity decreased by 20.8% and superoxide dismutase increased by 1.7 times. The proposed approach leads to an increase in the viability of experimental chickens and acceleration of embryogenesis.