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Glucose provides fuel for cell metabolism and plays an important role in normal reproductive function. Glucose transporters such as (SLC2A1) facilitate entry of glucose into cells. We have previously shown that gonadotropin releasing hormone (GnRH) stimulates Slc2a1 expression in LT2 gonadotrope cells and in the pituitary gland of pre-pubertal mice. In this study we examined changes in Slc2a1 expression in the pituitary gland of mice after puberty and in LT2 gonadotrope cells treated with sex steroids. There were no detectable changes in Slc2a1 gene expression in the pituitary gland of pre-pubertal and adult male and female mice using real-time PCR analysis. In contrast, Western blot analysis detected higher levels of SLC2A1 protein in the pituitary gland of adult male and female mice compared to pre-pubertal male and female mice, respectively.
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