This study aims to identify the capacity of two fluorescent Pseudomonas plant growth-promoting rhizobacteria and one strain of P. aeruginosa to produce biosurfactants. The strains were grown in Mineral Salt Medium (MSM) with 1% (v/v) tapis gas oil as a sole carbon and energy source. Biosurfactant synthesis was monitored by measuring surface tension, emulsifying index (E24), drop-collapse and bacterial adhesion to hydrocarbons. The best strain, P. aeruginosa, was able to reduce the surface tension to 55 mNm-1 with an E24 of 25.29%. The hydrophobicity was below 30%, a significant decrease for P. aeruginosa was signaled. Bacterial adhesion to hydrocarbons assay results showed that, P. aeruginosa had the highest level of cell adhesion (25.4%), followed by P. fluorescens (22.90%) and P. putida (17.07%). Temperature of 30°C and pH 7 were found to be optimum. This study showed that these PGPR strains had the ability to biodegrade gas oil and concurrently produce biosurfactant.