alexa Enhanced Intestinal Permeability of Tinospora cordifolia Extract Through Nanoemulsion Formulation: In-Vitro and Ex-Vivo Studies

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Enhanced Intestinal Permeability of Tinospora cordifolia Extract Through Nanoemulsion Formulation: In-Vitro and Ex-Vivo Studies

Clinical efficacy of phytotherapeutics has been limited due to their poor stability, solubility and oral bioavailability. The present study focused on designing and development of lipid based nanoformulation for improved efficacy and oral bioavailability of phytotherapeutics of Tinospora cardifolia (TC) stem extract. Nanoemulsions were prepared by sonication-solvent evaporation method and optimized for encapsulation efficiency, release kinetics as well as stability parameters by pseudo-ternary phase diagrams. Clove oil was used as lipid phase while Span 80 and Tween 20 were used as lipophilic surfactant and hydrophilic surfactant in order to enhance drug loading and permeation across the intestinal membrane. Increasing concentration of span 80 resulted in increased droplet size, while the reverse effect was observed by increasing the co-surfactant concentration. Based on the droplet size and zeta potential an optimized formulation consisting of clove oil, Span 80, Tween 20 and ethanol (7.7:4:0.5:21 w/w) and loaded with 83.8% of TC stem extract. The mean particle size of formulated nanoemulsion was found within the range of 33.7 nm to 377 nm. The active components remained stable in the optimized nanoemulsion during study for 3 months. 79.4 ± 1.5% and 80.5 ± 0.9% of extract were released from solution and nanoemulsion after 12 h and 20 h, respectively. Approximately 3.95 fold enhancement in permeability coefficient of TC extract was noticed upon formulating it as nanoemulsion in comparison to control by everted gut model. These results revealed the successful application of SNEDDS for improved permeation of potential phytotherapeutics across GIT towards enhancement of therapeutic efficacy.

 
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