alexa Regeneration of Kenyan Cassava (Manihot Esculenta Crantz) Genotypes

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Regeneration of Kenyan Cassava (Manihot Esculenta Crantz) Genotypes

A reproducible regeneration system based on direct somatic embryogenesis is described for Kenya cassava lines. Cassava plants were regenerated at high frequency by inducing shoot primordial on explants derived from cotyledons of cassava somatic embryos.Various parameters were evaluated on their effects on callus induction, somatic embryogenesis, maturation and germination of somatic embryos as well as recovery of regenerated plantlets. Immature leaf lobes were used as explants for somatic embryogenesis. Three Kenyan cassava genotypes viz; Adhiambo Lera, Kibanda Meno and Serere along with a model cultivar, TMS 60444 were used this system. Remarkable regeneration frequencies were observed in all the evaluated genotypes with Adhiambo Lera showing the best responses. As a result, a highly efficient plant regeneration protocol via germination of somatic embryos was achieved. This system enriches the scope of in vitro regeneration protocols for cassava and is envisaged to be a reliable prerequisite to genetic transformation of African cassava genotypes.

For more information : Ngugi MP, Oduor RO, Omwoyo RO, Njagi JM, Mgutu AJ et al. (2015) Regeneration of Kenyan Cassava (Manihot Esculenta Crantz) Genotypes. J Plant Biochem Physiol 3:147

  • Share this page
  • Facebook
  • Twitter
  • LinkedIn
  • Google+
  • Pinterest
  • Blogger