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Ayurvedic formulations [such as solid dosage (vati, churna), semisolid (avaleha, ghritas), liquid (asava, arishta)] have numerous uses in Ayurveda. They effect or help to rectify the three doshas in the body, and restore homeostatic balance that builds up in the body’s digestive system and spreads to the tissues. Standardization and analysis of the chemical marker of the Ayurvedic and other poly herbal formulation has always been a concern. For Researchers, Standardization is the need of hour for the present era to set standards for maintaining the quality and efficacy of the herbal products. HPTLC offers major advantages over other conventional chromatographic techniques such as unsurpassed flexibility (esp. stationary and mobile phase), choice of detection wavelength, user friendly, rapid and cost effective/economical.