In this new era of personalized cancer care, molecular testing for somatic mutations plays an increasing role in treatment decisions for many targeted cancer therapeutics. Formalin-fixed paraffin-embedded tissue (FFPET) specimens remain the typical source of nucleic acid for such testing. Because formalin fixation can damage nucleic acids and many tumor specimens are small, obtaining a sufficient quantity of high-quality DNA for mutation testing can be challenging.
Citation: Malhotra KT, Gulati U, Balzer B, Wu HY (2012) Comparison of DNA Extraction Methods from Formalin-Fixed, Paraffin-Embedded Tissue and their Impact on Real-Time PCR-Based Mutation Assays. J Med Diagn Meth 1:107. doi: 10.4172/2168-9784.1000107