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The transgenic lines and nontransgenic progenitor isoline (control) were used for both proteomic and phenolic compound analysis. Seed proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Out of approximately 1300 protein spots detected per protein extract, 30 spots were selected for further analysis based on software-determined differences (ANOVA) in their relative abundance in the protein gels for the control and three events. Subsequent statistical analysis after Bonferroni correction indicated that the abundance of only two of the thirty protein spots were significantly different at the 1% probability level.
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