Research studies in the heterologous expression of exogenous genes in living host cells are not new and have been ongoing since the first recombinant DNA molecule was created in the 1970’s. Conventionally, molecular cloning to produce recombinant DNA very often revolve around cleavage of chromosomal DNA or polymerase chain reaction (PCR)-amplified DNA molecules using restriction enzymes and insertion of the DNA fragments of interest with vector DNA, such as plasmids, bacmids or cosmids, with DNA ligase. The recombinant DNA that is constructed is then delivered or transformed into host cells for expression.
Chee JY, Chin CF (2015) Gateway Cloning Technology: Advantages and Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138