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In this study, the effects of four rice varieties including Gohar, Khazar, Kazemi and Hashemi were studied on digestive enzymatic activities and some components involved in intermediary metabolism of Chilo suppressalis Walker, the most important pest of rice in Iran. Activities of general protease, elastase and chymotrypsin showed statistical differences among rice varieties but no significant differences were found in case of other proteases. Feeding of larvae on the rice varieties showed the lowest activity of glucosidases and alpha-amylase in the larvae fed on Kazemi and Gohar, respectively. No statistical differences were found in lipase activity as activities of ALT, ACP and ALP. The highest activities of AST and LDH were found in the larvae fed on Hashemi. These results may helpful to have a background regarding planted varieties in the infested rice fields to C. suppressalis.