In grade to obtain high quality and safety food along with the environment protection from pesticides hazards, four bio agents were used to control fruit rot disease caused by Botrytis cinerea in strawberry fruits. Chlorella vulgris, Spirulina platensis, Azotobacter chroococcum, Trichoderma harizianum and their combinations (T1, T2, T3, T4, T12) were used as spray treatment in the open field and on strawberry after picking. The individual bio agents treatments decreased liner growth and spore production of B. cinerea in comparing with mixed bio agents. Also, all the treatments decreased Disease Severity% (DS%). The treatments of T1, T2, T3 and T4 caused 13.9, 22.3, 22.3 and 24.1 DS% during 2014; 12.26, 15.5, 17.0 and 21.86 DS% during 2015 in comparing with the mixed bio agents (T12) which caused 29.3 and 29.03 DS% during 2014 and 2015, respect., after three sprayings in the field. Also, the treatments of T1, T2, T3 and T4 caused 0.00 DS% after 2nd spray through the storage at 5?C. The treatment of T12 caused the highest increase in the total sugar content in strawberry fruits which infected naturally with B. cinerea and stored at 5°C for three weeks increased with T12. In addition, the treatment of T12 caused the highest increase in polyphenol oxidase (PPO) activity and peroxidase (PO) which were 0.459 and 0.360 U/mg fresh weight respect., in comparing with T1, T2, T3 and T4 which caused 0.278, 0.287, 0.298, 0.313 and 0.139, 0.202, 0.276, 0.302 U/mg fresh weight respect., after storage for 6 weeks. The treatment of T1 gave the best result in K% in leaves (1.82). T5 gave the best value of N% in soil (72.2%), T3 gave the best one in P% in soil (36.0%) and T4 caused the best amount of K% (16.0) in the soil.

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