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Microparticles (MPs) are defined a heterogeneous population of vesicles (diameter 100-1000 nm) that are released by cellular vesiculation and fission of the membrane of cells and play a pivotal role in various diseases including cardiovascular diseases, cancer, sepsis, eclampsia, autoimmune and metabolic states. Currently there is no standardization regarding analytical methods of MP detection. Conventional methods have crucial limitations regarding complicated assay and suffers from relatively low sensitivity and accuracy because of resolution problems occurring for the majority of commercially available flow cytometers. Alternatively, recently recognized as a method for quantification and sizing of biological nanoparticles surface plasmon resonance-based imaging microscopy (SPRi microscopy) might be significantly useful to resolve the majority problems affected MPs recognition. Probably Raman micro-spectroscopy, micro nuclear magnetic resonance technique, small-angle X-ray scattering, and anomalous small-angle X-ray scattering might compete with SPRi microscopy and flow cytometery. Sort comment is discussed contemporary approaches regarding novel techniques of microparticle determination, measurements and assay.
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